Project Overview
The role of Klf4 in genomic stability
Department(s)
Biology
Abstract
Kru¨ppel-like factor 4 (Klf4) is a zinc-finger-containing transcription factor with tumor suppressor activity that is mutated in various cancer types including colorectal cancer. Despite ample contextual evidence of Klf4’s role in cancer development, the specific molecular mechanisms targeted are not fully understood. Our research projects will determine the impacts of altered expression and function of Klf4 on ferroptosis (a form of regulated cell death caused by lipid peroxidation) pathways in relation to Klf4 and cancer metabolism in mouse embryonic fibroblast cells (MEFs) and colorectal, RKO cancer cell lines. We will examine the downstream molecules involved in these processes. The research students will integrate several different techniques, including, but not limited to, gene transfection, culture of primary cells, cell proliferation and metabolism assays, protein extraction and immunoblot analysis.
Student Qualifications
Students must complete Biology 182. Tiisue culure experience is preferable, but not required.
Number of Student Researchers
2 students
Project Length
10 weeks
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