Project Overview

Stress hormone signaling crosstalk in Arabidopsis

Faculty Sponsor

Raj Mohan (rmohan@colgate.edu)

Department(s)

Biology

Abstract

Plants use distinct stress hormone signals in response to biotic stresses like pathogens and abiotic stresses like wounding. The goal of this project is to identify the mechanism by which these signaling pathways interact in the model plant, Arabidopsis. Preliminary evidence in our lab shows that pathogen stress hormone receptors can activate a non-canonical pathway to promote wound response. Students will test the hypothesis whether plants with acquired immunity also show improved wound healing. Students will use molecular, genetic, biochemical strategies to determine the mechanism by which these signaling pathways interacts. Possible techniques include quantitative RealTime PCR to study gene expression, Western blotting to study protein levels and phosphorylation, in vitro degradation assays to study proteasome-mediated degradation, fluorescence microscopy to visualize GFP-tagged proteins, gene cloning and transformation and plant physiological assays. 

Student Qualifications

Basic biology lab experience preferred (e.g. 182 lab)

Number of Student Researchers

3 students

Project Length

7 weeks


Applications open on 01/05/2019 and close on 02/05/2019


<< Back to List





If you have questions, please contact Karyn Belanger (kgbelanger@colgate.edu).